ABRF-sPRG 2020: Single Injection Focused Information Acquisition of a Secure Isotope Labeled Phosphopeptide Customary
The mission of the ABRF Proteomics Requirements Analysis Group (sPRG) is to determine and implement technical requirements that mirror the ABRF’s dedication to accuracy, readability, and consistency within the discipline of proteomics. There may be broad curiosity in quantifying protein phosphorylation alterations in mobile signaling pathways, however their transient nature and low abundance makes their evaluation difficult. Right here we comply with up on our sPRG heavy-labeled phosphopeptide normal research designed to deal with points encountered in phosphopeptide experiments.
This normal is constructed of 150 heavy-labeled phosphopeptides spanning seven totally different signaling pathways and will probably be helpful to check the effectiveness of phosphopeptide enrichment workflows, as an inside instrument and chromatography calibrant, and as a pre-built organic assay for all kinds of signaling pathways. In our preliminary characterization and validation of this normal (sPRG 2018-2019 research), we blended the usual into an activated HeLa tryptic digest and distributed the combination to individuals with optimized and standardized enrichment and acquisition strategies.
Information impartial acquisition (DIA) was carried out on eight gasoline part fractionated injections. As an extension of this preliminary characterization and to organize for a bigger organic research, we current progress in direction of a common single inject focused information acquisition methodology utilizing our normal which goals to be correct, delicate, scalable, and simply carried out and executed with out the necessity for retention time scheduling.
Two approaches had been taken that each goal the heavy isotope labeled inside normal peptides and set off a mass offset scan of the endogenous gentle. The primary methodology, QuanDirect, screens for heavy y1 fragments and might be carried out on tribrid instrument platforms. The second methodology, SureQuant, screens for choose fragment ions and might be carried out on each tribrid and hybrid devices.
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Rat Anti-Mouse IL-12 p75 Monoclonal antibody, clone R2-9A5
Description: TNFRSF10B (Tumor necrosis factor receptor superfamily, member 10b) has many names, including DR5, CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL R2, KILLER/DR5. The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces apoptosis signal. Mice have a homologous gene, tnfrsf10b, that has been essential in the elucidation of the function of this gene in humans. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. By analysis of radiation hybrid panels, Walczak et al.(1997) mapped the gene to chromosome 8p22-p21. Northern blot analysis indicated that TRAIL R2 was expressed as a 4.4-kb mRNA in all tissues tested, with the highest levels of expression in peripheral blood lymphocytes, spleen, and ovary.
Description: TNFRSF10B (Tumor necrosis factor receptor superfamily, member 10b) is a human gene. It is also known as DR5 (Death Receptor 5), CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL-R2, KILLER/DR5. The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces apoptosis signal. Mice have a homologous gene, tnfrsf10b that has been essential in the elucidation of the function of this gene in humans. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.By analysis of radiation hybrid panels, this gene is mapped to chromosome 8p22-p21. Northern blot analysis indicated that TRAILR2 was expressed as a 4.4-kb mRNA in all tissues tested, with the highest levels of expression in peripheral blood lymphocytes, spleen, and ovary.
Description: TNFRSF10B (Tumor necrosis factor receptor superfamily, member 10b) is a human gene. It is also known as DR5, CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL-R2, KILLER/DR5. The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces apoptosis signal. Mice have a homologous gene, tnfrsf10b that has been essential in the elucidation of the function of this gene in humans. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.By analysis of radiation hybrid panels, this gene is mapped to chromosome 8p22-p21. Northern blot analysis indicated that TRAILR2 was expressed as a 4.4-kb mRNA in all tissues tested, with the highest levels of expression in peripheral blood lymphocytes, spleen, and ovary.
Description: The protein encoded by the TNFRSF10B is a member of the TNF-receptor superfamily, and contains an intracellular death domain. Death receptor 5 can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. [RefSeq]
Description: Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated by two distinct cell surface receptors, designated TNF-R1 and TNF-R2, which are expressed on most cell types. TNF function is primarily mediated through TNF-R1 signaling. Both receptors belong to the growing TNF receptor superfamily which includes Fas antigen and CD40. TNF-R1 contains a cytoplasmic motif, termed the death domain, that has been found to be necessary for the transduction of the apoptotic signal. The death domain is also found in several other receptors, including Fas, DR2 (or TRUNDD), DR3 (death receptor 3), DR4 and DR5. TRUNDD, DR4 and DR5 are receptors for the apoptosis-inducing cytokine TRAIL. A non-death domain-containing receptor, designated decoy receptor (DcR1 or TRID), also specifically associates with TRAIL and may play a role in cellular resistance to apoptotic stimuli.
Description: Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated by two distinct cell surface receptors, designated TNF-R1 and TNF-R2, which are expressed on most cell types. TNF function is primarily mediated through TNF-R1 signaling. Both receptors belong to the growing TNF receptor superfamily which includes Fas antigen and CD40. TNF-R1 contains a cytoplasmic motif, termed the death domain, that has been found to be necessary for the transduction of the apoptotic signal. The death domain is also found in several other receptors, including Fas, DR2 (or TRUNDD), DR3 (death receptor 3), DR4 and DR5. TRUNDD, DR4 and DR5 are receptors for the apoptosis-inducing cytokine TRAIL. A non-death domain-containing receptor, designated decoy receptor (DcR1 or TRID), also specifically associates with TRAIL and may play a role in cellular resistance to apoptotic stimuli.
Description: Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated by two distinct cell surface receptors, designated TNF-R1 and TNF-R2, which are expressed on most cell types. TNF function is primarily mediated through TNF-R1 signaling. Both receptors belong to the growing TNF receptor superfamily which includes Fas antigen and CD40. TNF-R1 contains a cytoplasmic motif, termed the death domain, that has been found to be necessary for the transduction of the apoptotic signal. The death domain is also found in several other receptors, including Fas, DR2 (or TRUNDD), DR3 (death receptor 3), DR4 and DR5. TRUNDD, DR4 and DR5 are receptors for the apoptosis-inducing cytokine TRAIL. A non-death domain-containing receptor, designated decoy receptor (DcR1 or TRID), also specifically associates with TRAIL and may play a role in cellular resistance to apoptotic stimuli.
Description: A polyclonal antibody for detection of TNF-R2 from Human, Mouse, Rat. This TNF-R2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human TNF-R2 at AA range: 350-430
Description: A polyclonal antibody for detection of TNF-R2 from Human, Mouse, Rat. This TNF-R2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human TNF-R2 at AA range: 350-430
Description: A polyclonal antibody for detection of TNF-R2 from Human, Mouse, Rat. This TNF-R2 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human TNF-R2 at AA range: 350-430
Description: A polyclonal antibody for detection of GABAB R2 from Human, Mouse, Rat. This GABAB R2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human GABAB R2 around the non-phosphorylation site of S893
Description: A polyclonal antibody for detection of GABAB R2 from Human, Mouse, Rat. This GABAB R2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human GABAB R2 around the non-phosphorylation site of S893
Description: A polyclonal antibody for detection of GABAB R2 from Human, Mouse, Rat. This GABAB R2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human GABAB R2 around the non-phosphorylation site of S893
Description: This monoclonal antibody enables sensitive and specific detection of human IL-2 in immunoassays such as ELISA and ELISpot. The antibody is also suitable for detection of IL-2 using Flow cytometry.
Description: This monoclonal antibody enables sensitive and specific detection of human IL-17A in immunoassays such as ELISA and ELISpot. The antibody is also suitable for detection of IL-17A using Flow cytometry.
Description: This monoclonal antibody enables sensitive and specific detection of human IL-1β in immunoassays such as ELISA and ELISpot. The antibody binds to the pro-form and the active form of IL-1β. It does not cross-react with IL-1α.
Description: This monoclonal antibody enables sensitive and specific detection of human IL-1β in immunoassays such as ELISA and ELISpot. The antibody binds to the pro-form and the active form of IL-1β. It does not cross-react with IL-1α.
Description: This monoclonal antibody enables sensitive and specific detection of human IL-1α in immunoassays such as ELISA, ELISpot, and Western blot.
Description: Description of target: Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 32 pg/mL
Description: Description of target: This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 16 pg/mL
Description: Description of target: The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 8 pg/mL
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.
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Willpower of arginine δ15N values in plant and animal proteins by gasoline chromatography-combustion-isotope ratio mass spectrometry
Nitrogen secure isotope strategies are extensively utilized in ecology, archaeology and forensic science to discover trophic relation-ships and provenances of organisms and supplies, most generally utilizing bulk δ15N values of complete organisms, tissues or different supplies. Nevertheless, compound-specific isotope values can present extra diagnostic isotope “fingerprints” and particular in-formation about metabolic processes. Current strategies for nitrogen isotope evaluation enable the dedication of δ15N val-ues of 14 amino acids (AAs), accounting for ca. 75% of plant protein and collagen N.
The vast majority of remaining N is from arginine, comprising 16% and 14% of collagen and plant protein N, respectively. We subsequently aimed to develop a way to detect arginine and decide its δ15N worth (δ15NArg) by GC-C-IRMS, to additional contribute to the understanding of meta-bolic routing of this vital AA.
We display that arginine, as its N-acetyl isopropyl ester, is amenable to GC evaluation utilizing a 15 m mid-polarity DB 35 column, eluting with baseline decision from different AAs. The recorded δ15N worth by GC C IRMS was inside error of that of the underivatized compound decided by EA-IRMS. The newly-developed GC C IRMS methodology was utilized to trendy plant protein and cattle collagen, enabling their δ15NArg values to be associated to AA biosynthesis.
Determinations of archaeological cattle collagen δ15NArg values confirmed the suitability of this methodology to offer additional insights into previous diets and ecosystems. Bulk collagen δ15N worth reconstruction together with δ15NArg values bet-ter mirror measured bulk values, because the isotopic ratio of 91‰ of collagen N can now be decided on the compound-specific stage.
Comparative Research of the Results of Salinity on Development, Gasoline Trade, N Accumulation and Secure Isotope Signatures of Forage Oat ( Avena sativa L.) Genotypes
Figuring out appropriate salt stress-tolerant phenotypes based mostly on their agronomic and physiological traits stays a herculean activity in forage-type oat (Avena sativa L.) breeding. This research examined the responses of six forage-type oat cultivars below 4 ranges of saline stress over the vegetative development cycle.
Crop development, water status-related traits and nitrogen status-related traits had been analyzed in several plant components to guage efficient approaches for figuring out salt tolerance. Plant biomass, top, tiller quantity and culm thickness modified considerably throughout salinity, however they weren’t exact sufficient to be used in estimating genotypic salinity tolerance throughout long-term stress. Genotypes bearing bigger numbers of tillers confirmed higher sensitivity to salinity on account of its results on biomass loss.
Tolerant genotypes exhibited larger relative shoot biomass along with larger water use effectivity. The concentrations of Na+, Ok+ and their ratio, mixed with the δ13C in shoots and roots had been efficient indicators for estimating tolerant genotypes by higher water upkeep.
N concentrations of shoots had been probably the most environment friendly for evaluating genotypic tolerance. Low nitrate reductase (NR) and glutamine synthetase (GS) exercise may be key components limiting N accumulation. Chlorophyll (Chl) content material and web photosynthetic price, in addition to stomatal conductance and evaporation, had been helpful for figuring out salinity tolerance physiological mechanisms, however the effectiveness was low for genotypic tolerance testing for forage sort oats because of the interplay between genotypes and salinity ranges.
The number of excessive salinity-tolerant genotypes ought to give attention to genotypes with photosynthetic resilience to salt, adopted by excessive N metabolism (larger NR and GS actions) to make sure accumulation of extra N within the shoot dry matter.
Description: Recombinant human Interleukin-8 is a disulfide-linked monomer protein consisting of 78 amino acid residues, migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-8 mature chain was expressed in E. coli.
Description: Recombinant human Interleukin-8 is a disulfide-linked monomer protein consisting of 78 amino acid residues, migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-8 mature chain was expressed in E. coli.
Description: Quantitative sandwich ELISA kit for measuring Human Interleukin 8, IL-8 in samples from serum, cell culture supernates, saliva, urine, cerebrospinalfluid (CSF), tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative sandwich ELISA kit for measuring Human Interleukin 8, IL-8 in samples from serum, cell culture supernates, saliva, urine, cerebrospinalfluid(CSF), tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Interleukin-8 (IL-8), also known as CXCL8, is an ELR-positive CXC family member chemokine produced by macrophages and other cell types such as epithelial cells. ELR-positive CXC chemokines such as IL-8 specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. Human IL-8 Recombinant Protein is purified interleukin-8 produced in yeast.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Interleukin 8,IL-8 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: IL8 is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor.
Description: IL8 is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor.
Description: Interleukin-8(IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the IL8 gene. Interleukin-8(IL8) is a member of the CXC chemokine family(Hull et al., 2001). IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. The genes for IL8 have been co-localized on a 335-kb genomic fragment.
Description: Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the IL8 gene. Interleukin-8 (IL8) is a member of the CXC chemokine family. IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. The genes for IL8 have been co-localized on a 335-kb genomic fragment.
Description: Quantitative sandwich ELISA for measuring Human IL-8 in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human IL-8 in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human IL-8 in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Interleukin-8 (IL-8) is a proinflammatory CXC chemokine produced by macrophages, epithelial cells. IL-8 is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies
Description: Interleukin-8 (IL-8) is a proinflammatory CXC chemokine produced by macrophages, epithelial cells. IL-8 is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies
Description: Description of target: Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils.1 IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications.2 The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside.1 Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 1 pg/ml
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IL-8 . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IL-8
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IL-8
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IL-8
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IL-8
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IL-8
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IL-8
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human IL-8
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human IL-8
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human IL-8
Description: Interleukin-8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 77 amino acids and having a molecular mass of 8904 Dalton. ;The IL-8 is purified by proprietary chromatographic techniques.
Description: Description of target: The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 16 pg/mL
Description: IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (monocyte-derived) is an 8.4 kDa protein containing 72 amino acid residues.
Description: IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (endothelial-derived) is an 8.9 kDa protein containing 77 amino acid residues.
Description: Interleukin-8 Human Recombinant produced in Yeast is a single, glycosylated polypeptide chain containing 79 amino acids and having a molecular mass of 9 kDa. ;The IL-8 is purified by proprietary chromatographic techniques.
IL-8 Interleukin-8 (1-72 a.a.) HumanRecombinant Protein (CXCL8)
Description: Interleukin-8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 72 amino acids and having a molecular mass of 8452 Dalton. ;The IL-8 is purified by proprietary chromatographic techniques.
Description: IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (monocyte-derived) is an 8.4 kDa protein containing 72 amino acid residues.
Description: IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (monocyte-derived) is an 8.4 kDa protein containing 72 amino acid residues.
Description: Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 levels are associated with the onset of a variety of disease states.
Description: Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 levels are associated with the onset of a variety of disease states.
Description: Interleukin-8 (IL-8) was originally discovered as a neutrophil chemotactic and activating factor and is a member of the alpha (CXC) subfamily of chemokines (including also platelet factor 4, GRO, IP-10, etc.). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes and various tumor cell lines, produce IL-8 in response to a wide variety of proinflammatory stimuli such as exposure to IL-1, TNF, LPS and viruses. IL-8 has a wide range of other proinflammatory effects. It is a potent chemoattractant for neutrophils and causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and subendothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. IL-8 was also reported to be angiogenic both in vivo and in vitro.
Description: Interleukin-8 (IL-8) was originally discovered as a neutrophil chemotactic and activating factor and is a member of the alpha (CXC) subfamily of chemokines (including also platelet factor 4, GRO, IP-10, etc.). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes and various tumor cell lines, produce IL-8 in response to a wide variety of proinflammatory stimuli such as exposure to IL-1, TNF, LPS and viruses. IL-8 has a wide range of other proinflammatory effects. It is a potent chemoattractant for neutrophils and causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and subendothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. IL-8 was also reported to be angiogenic both in vivo and in vitro.
